Molecular characterisation of In51, a class 1 integron containing a novel aminoglycoside adenylyltransferase gene cassette, aadA6, in Pseudomonas aeruginosa

T Naas; L Poirel; P Nordmann

doi:10.1016/s0167-4781(99)00202-x

Molecular characterisation of In51, a class 1 integron containing a novel aminoglycoside adenylyltransferase gene cassette, aadA6, in Pseudomonas aeruginosa

Biochim Biophys Acta. 1999 Dec 23;1489(2-3):445-51. doi: 10.1016/s0167-4781(99)00202-x.

Authors

T Naas¹, L Poirel, P Nordmann

Affiliation

¹ Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique-Hôpitaux de Paris, Le Kremlin-Bicêtre, France. [email protected]

PMID: 10673049
DOI: 10.1016/s0167-4781(99)00202-x

Abstract

Polymerase chain reaction-amplification and subsequent sequencing of the variable region of a novel integron, In51, from Pseudomonas aeruginosa revealed the presence of a novel aminoglycoside adenylyltransferase gene, aadA6, together with an open reading frame of unknown function, orfD. AADA6 enzyme has only 75% amino acid identity with AADA1 and is able to confer high level resistance to streptomycin and spectinomycin in Escherichia coli.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacterial Proteins / genetics*
Base Sequence
DNA, Bacterial
Molecular Sequence Data
Nucleotidyltransferases / genetics*
Pseudomonas aeruginosa / enzymology
Pseudomonas aeruginosa / genetics*
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid

Substances

Bacterial Proteins
DNA, Bacterial
Nucleotidyltransferases
aadA6 protein, Pseudomonas aeruginosa

Associated data

GENBANK/AF140629