Abstract
Clostridium botulinum type A hemagglutinin-positive progenitor toxin consists of three distinct components: neurotoxin (NTX), hemagglutinin (HA), and non-toxic non-HA (NTNH). The HA consists of four subcomponents designated HA1, 2, 3a and 3b. By employing purified toxin and GST-fusion proteins of each HA subcomponent, we found that the HA-positive progenitor toxin, GST-HA1 and GST-HA3b bind to human erythrocytes and microvilli of guinea pig upper small intestinal sections. The HA-positive progenitor toxin and GST-HA1 bind via galactose moieties, GST-HA3b binds via sialic acid moieties. GST-2 and GST-3a showed no detectable binding.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / chemistry
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Bacterial Proteins / metabolism
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Botulinum Toxins*
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Botulinum Toxins, Type A / chemistry*
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Botulinum Toxins, Type A / metabolism
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Clostridium botulinum*
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Erythrocytes / metabolism
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Galactose / metabolism
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Guinea Pigs
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Hemagglutinins / chemistry*
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Hemagglutinins / genetics
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Hemagglutinins / metabolism
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Intestine, Small / metabolism
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Microvilli / metabolism
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N-Acetylneuraminic Acid / metabolism
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Recombinant Fusion Proteins / metabolism
Substances
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Bacterial Proteins
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Hemagglutinins
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NTNH protein, Clostridium botulinum
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Recombinant Fusion Proteins
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Botulinum Toxins
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Botulinum Toxins, Type A
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N-Acetylneuraminic Acid
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Galactose