Reinitiation of protein synthesis in Escherichia coli can be induced by mRNA cis-elements unrelated to canonical translation initiation signals

A Andrè; A Puca; F Sansone; A Brandi; G Antico; R A Calogero

doi:10.1016/s0014-5793(00)01198-4

Reinitiation of protein synthesis in Escherichia coli can be induced by mRNA cis-elements unrelated to canonical translation initiation signals

FEBS Lett. 2000 Feb 18;468(1):73-8. doi: 10.1016/s0014-5793(00)01198-4.

Authors

A Andrè¹, A Puca, F Sansone, A Brandi, G Antico, R A Calogero

Affiliation

¹ Dipartimento di Genetica, Biologia Generale e Molecolare, Università Federico II di Napoli, via Mezzocannone 8, 80134, Naples, Italy.

PMID: 10683444
DOI: 10.1016/s0014-5793(00)01198-4

Abstract

In Eubacteria, de novo translation of some internal cistrons may be inefficient or impossible unless the 5' neighboring cistron is also translated (translational coupling). Translation reinitiation is an extreme case of translational coupling in which translation of a message depends entirely on the presence of a nearby terminating ribosome. In this work, the characteristics of mRNA cis-elements inducing the reinitiation process in Escherichia coli have been investigated using a combinatorial approach. A number of novel translational reinitiation sequences (TRSs) were thus identified, which show a wide range of reinitiation activities fully dependent on a translational coupling event and unrelated to the presence/absence of secondary structure or mRNA stability. Moreover, some of the isolated TRSs are similar to intercistronic sequences present in the E. coli genome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

5' Untranslated Regions / genetics
ATP-Binding Cassette Transporters*
Bacterial Proteins / biosynthesis*
Bacterial Proteins / genetics
Carrier Proteins / biosynthesis
Cell-Free System / metabolism
Codon, Initiator / genetics*
Codon, Terminator / genetics
Escherichia coli / enzymology
Escherichia coli / genetics*
Escherichia coli Proteins*
Genes / genetics
Genes, Reporter
Genetic Vectors
Glutathione Transferase / biosynthesis
Glutathione Transferase / genetics
Maltose-Binding Proteins
Monosaccharide Transport Proteins*
Protein Biosynthesis*
RNA, Messenger / genetics*
RNA, Messenger / metabolism
Signal Transduction / genetics
Transformation, Bacterial
beta-Galactosidase / biosynthesis
beta-Galactosidase / genetics

Substances

5' Untranslated Regions
ATP-Binding Cassette Transporters
Bacterial Proteins
Carrier Proteins
Codon, Initiator
Codon, Terminator
Escherichia coli Proteins
Maltose-Binding Proteins
Monosaccharide Transport Proteins
RNA, Messenger
maltose transport system, E coli
Glutathione Transferase
beta-Galactosidase