Abstract
Studies of the early stages of T-cell activation reveal that T cells from aged mice show multiple abnormalities within the first few minutes after stimulation, including decline in the activation of the Raf-1/MEK/ERK kinases and in JNK protein kinase. Zap-70 kinase associated with the CD3zeta chain shows a 2-fold increase with age in resting CD4 T cells, despite a three-fold decline with age in the levels of tyrosine phosphorylation of CD3zeta; nonetheless, there is no effect of aging on Zap-70 kinase function in activated T cells as measured by in vitro kinase methods. Age-related impairment of the translocation of PKCθ from cytoplasm to the site of T-cell interaction with antigen-presenting cells may underlie downstream defects in the activation cascade.
Publication types
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Aging / immunology*
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Animals
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Antibody-Producing Cells / immunology
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Antigens
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CD3 Complex / metabolism
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Humans
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Isoenzymes / metabolism
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JNK Mitogen-Activated Protein Kinases
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Lymphocyte Activation*
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Mice
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Mitogen-Activated Protein Kinases / metabolism
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Protein Kinase C / metabolism
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Protein Kinase C-theta
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Protein Kinases / metabolism
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Protein-Tyrosine Kinases / metabolism
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Proto-Oncogene Proteins c-raf / metabolism
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T-Lymphocytes / enzymology
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T-Lymphocytes / immunology*
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ZAP-70 Protein-Tyrosine Kinase
Substances
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Antigens
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CD3 Complex
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Isoenzymes
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Protein Kinases
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Protein-Tyrosine Kinases
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ZAP-70 Protein-Tyrosine Kinase
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ZAP70 protein, human
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Zap70 protein, mouse
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Proto-Oncogene Proteins c-raf
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PRKCQ protein, human
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Prkcq protein, mouse
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Protein Kinase C
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Protein Kinase C-theta
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JNK Mitogen-Activated Protein Kinases
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Mitogen-Activated Protein Kinases