Rapid separation of lysozyme from chicken egg white by reductants and thermal treatment

J Agric Food Chem. 2000 Feb;48(2):161-4. doi: 10.1021/jf9902797.

Abstract

Reductants (0.1-2.0% ascorbic acid, cysteine, or cystine and 0.04-1. 0% beta-mercaptoethanol) were added to 5-fold diluted, salted duck egg whites (commercially and laboratory prepared) and fresh egg whites (chicken and duck), and subsequently the mixtures were heated at 70 degrees C for 1-10 min. The maximal recovery and purification fold of lysozyme obtained from fresh chicken egg whites added with 1. 0% ascorbic acid were 78% and 2.4, respectively. Storage tests showed that the obtained lyophilized lysozyme powder after dialysis was stable when refrigerated at 4 degrees C for 3 months.

MeSH terms

  • Animals
  • Chickens
  • Ducks
  • Egg White / analysis*
  • Hot Temperature
  • Muramidase / isolation & purification*

Substances

  • Muramidase