Exocytosis and endocytosis in juxtaglomerular cells

Acta Physiol Scand. 2000 Jan;168(1):95-9. doi: 10.1046/j.1365-201x.2000.00645.x.

Abstract

The cellular events related to secretion of renin are not well understood. Here we review some of the evidence that has led to the current understanding of renin secretion as a process that involves exocytosis as the predominant mode of secretion. This is based on the observation of occasional fusion events between secretory granules and cell membrane and measurement of intermittent secretion of renin from single afferent arterioles, with a renin content of each secretion episode that corresponds to the renin content of one secretory granule. More recently it has been demonstrated that the afferent arterioles lose a large number of renin granules after acute stimulation without changing the average granular volume. Current electrophysiological techniques have now permitted direct measurements of cell membrane capacitance in juxtaglomerular (JG) cells as a measure of net addition (exocytosis) or removal (endocytosis) of membrane material. With this technique we have shown that cAMP, which is a vasodilator and stimulates renin secretion, enhances net exocytosis at low concentrations, while at higher concentrations membrane retrieval processes are also stimulated. We suggest that both exocytosis and endocytosis are regulated processes in the JG-cells and both may be important for the long-term control of renin secretion at the single cell level.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cyclic AMP / pharmacology
  • Electric Conductivity
  • Endocytosis / physiology*
  • Exocytosis / physiology*
  • Juxtaglomerular Apparatus / cytology
  • Juxtaglomerular Apparatus / drug effects
  • Juxtaglomerular Apparatus / metabolism
  • Juxtaglomerular Apparatus / physiology*
  • Patch-Clamp Techniques
  • Renin / metabolism

Substances

  • Cyclic AMP
  • Renin