Use of calibrated viral load standards for group M subtypes of human immunodeficiency virus type 1 to assess the performance of viral RNA quantitation tests

J Clin Microbiol. 2000 Mar;38(3):1247-9. doi: 10.1128/JCM.38.3.1247-1249.2000.

Abstract

Optimal management of human immunodeficiency virus type 1 (HIV-1) disease requires accurate quantitation of viral RNA concentrations in plasma. Evidence for increasing geographic intermixing of HIV-1 subtypes makes equivalent quantitation of all subtypes essential. The performances of six quantitative viral RNA tests are described, for the first time, with calibrated viral isolates of diverse subtypes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acquired Immunodeficiency Syndrome / blood
  • Acquired Immunodeficiency Syndrome / diagnosis*
  • Calibration
  • Geography
  • HIV-1 / classification*
  • HIV-1 / isolation & purification
  • Humans
  • RNA, Viral / blood*
  • Reproducibility of Results
  • Viral Load / methods*

Substances

  • RNA, Viral