Helminthic transmission and isolation of Ehrlichia risticii, the causative agent of Potomac horse fever, by using trematode stages from freshwater stream snails

N Pusterla; J E Madigan; J S Chae; E DeRock; E Johnson; J B Pusterla

doi:10.1128/JCM.38.3.1293-1297.2000

Helminthic transmission and isolation of Ehrlichia risticii, the causative agent of Potomac horse fever, by using trematode stages from freshwater stream snails

J Clin Microbiol. 2000 Mar;38(3):1293-7. doi: 10.1128/JCM.38.3.1293-1297.2000.

Authors

N Pusterla¹, J E Madigan, J S Chae, E DeRock, E Johnson, J B Pusterla

Affiliation

¹ Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California 95616, USA. [email protected]

Abstract

We report successful helminthic transmission of Ehrlichia risticii, the causative agent of Potomac horse fever, using trematode stages collected from Juga yrekaensis snails. The ehrlichial agent was isolated from the blood of experimentally infected horses by culture in murine monocytic cells and identified as E. risticii ultrastructurally and by characterization of three different genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Ehrlichia / genetics
Ehrlichia / isolation & purification*
Ehrlichia / ultrastructure
Ehrlichiosis / transmission
Ehrlichiosis / veterinary*
Female
Fresh Water
Helminths / microbiology*
Horse Diseases / transmission*
Horses
Macrophages / microbiology
Macrophages / ultrastructure
Male
Mice
Molecular Sequence Data
Orchiectomy
Polymerase Chain Reaction / methods
Snails / parasitology*
Vacuoles / microbiology

Associated data

GENBANK/AF179349
GENBANK/AF179350
GENBANK/AF179351