Abstract
By using radioimmunoassays toward the cDNA-predicted amino acid sequence of human glucagon-like peptide-2, a peptide was isolated from extracts of human ileum. By mass spectrometry and Edman sequencing, this peptide was identified as human proglucagon 126-158. High-performance liquid chromatography analyses indicated that a similar immunoreactive peptide (iGLP-2) was present in human plasma. Human plasma concentrations of iGLP-2 were elevated 3- to 4-fold at 1 to 2 h after ingestion of 800 to 1200 kcal meals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Chromatography, High Pressure Liquid
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Glucagon / analysis
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Glucagon / chemistry
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Glucagon / metabolism
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Glucagon-Like Peptide 2
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Glucagon-Like Peptides
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Humans
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Ileum / chemistry
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Mass Spectrometry
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Pancreas / chemistry
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Peptide Fragments / analysis
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Peptide Fragments / chemistry
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Peptide Fragments / metabolism
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Peptides / analysis
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Peptides / chemistry*
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Peptides / metabolism
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Proglucagon
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Protein Precursors / analysis
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Protein Precursors / chemistry
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Protein Precursors / metabolism
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Radioimmunoassay / methods
Substances
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Glucagon-Like Peptide 2
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Peptide Fragments
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Peptides
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Protein Precursors
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Proglucagon
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Glucagon-Like Peptides
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Glucagon