Abstract
CBA/J mice can be protected against lethal infection with Trypanosoma cruzi by treatment using T. cruzi soluble extract antigen (TCSE). In vivo administration of TCSE (400 microg/mouse) into naive mice increased the cellular proliferative response to Con A and elevated the levels of IFN-gamma. The production of IFN-gamma was extremely important in controlling the replication of the parasite since the protective activity of TCSE was completely abrogated by in vivo treatment with an anti IFN-gamma neutralizing antibody. These results suggest that depending on the level, cytokine production results in the control of replication of the parasite in experimental Chagas' disease.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Blocking / adverse effects
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Antibodies, Blocking / pharmacology
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Antigens, Protozoan / immunology
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Antigens, Protozoan / therapeutic use*
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Cell-Free System / immunology
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Chagas Disease / immunology*
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Chagas Disease / metabolism
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Chagas Disease / prevention & control*
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Concanavalin A / immunology
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Dose-Response Relationship, Immunologic
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Female
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Interferon-gamma / antagonists & inhibitors
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Interferon-gamma / immunology
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Interferon-gamma / metabolism
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Interferon-gamma / physiology*
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Lymphocyte Activation / immunology
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Mice
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Mice, Inbred CBA
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Protozoan Vaccines / antagonists & inhibitors
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Protozoan Vaccines / immunology
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Protozoan Vaccines / therapeutic use
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Solubility
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Spleen / cytology
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Spleen / immunology
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Spleen / metabolism
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Trypanosoma cruzi / immunology*
Substances
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Antibodies, Blocking
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Antigens, Protozoan
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Protozoan Vaccines
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Concanavalin A
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Interferon-gamma