Purification, crystallization and preliminary x-ray diffraction analysis of carboxyhaemoglobin-II from the fish Piaractus mesopotamicus (pacu)

V Fadel; R T Honda; M Dellamano; A L Smarra; P Delatorre; J R Olivieri; G O Bonilla-Rodriguez; W F de Azevedo Jr

doi:10.1107/s0907444900000585

Purification, crystallization and preliminary x-ray diffraction analysis of carboxyhaemoglobin-II from the fish Piaractus mesopotamicus (pacu)

Acta Crystallogr D Biol Crystallogr. 2000 Mar;56(Pt 3):366-7. doi: 10.1107/s0907444900000585.

Authors

V Fadel¹, R T Honda, M Dellamano, A L Smarra, P Delatorre, J R Olivieri, G O Bonilla-Rodriguez, W F de Azevedo Jr

Affiliation

¹ Departamento de Física, IBILCE-UNESP, CP 136, CEP 15054-000, São José do Rio Preto SP, Brazil.

PMID: 10713529
DOI: 10.1107/s0907444900000585

Abstract

Carboxyhaemoglobin-II isolated from the pacu (Piaractus mesopotamicus) has been crystallized and X-ray diffraction data were collected to 2.0 A resolution using synchrotron radiation. Crystals were characterized as belonging to the space group I23; preliminary structural analysis reveals the presence of one dimer in the asymmetric unit.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carboxyhemoglobin / chemistry*
Carboxyhemoglobin / isolation & purification
Crystallization
Crystallography, X-Ray
Cypriniformes / blood*
Models, Molecular
Protein Conformation

Substances

Carboxyhemoglobin