Abstract
Here, we describe the molecular and immunological characterization of the bdr gene family of Borrelia turicatae, a relapsing-fever spirochete. Nine bdr alleles belonging to two different subfamilies were sequenced and localized to linear plasmids. Anti-Bdr antiserum was generated and used to analyze Bdr expression in pre- and postinfection isogenic populations. The analyses presented here provide a detailed characterization of the Bdr proteins in a relapsing-fever spirochete species, enhancing our understanding of these proteins at the genus-wide level.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Amino Acid Sequence
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Animals
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Bacterial Proteins / chemistry*
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Borrelia / genetics*
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Escherichia coli / metabolism
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Evolution, Molecular
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Gene Library
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Immunoblotting
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Male
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Mice
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Mice, Inbred C3H
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Molecular Sequence Data
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Multigene Family
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Oligonucleotide Probes
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Open Reading Frames
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Plasmids
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Sequence Homology, Amino Acid
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Temperature
Substances
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Bacterial Proteins
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Oligonucleotide Probes
Associated data
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GENBANK/AF128445
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GENBANK/AF128446
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GENBANK/AF128447
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GENBANK/AF128448
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GENBANK/AF128449
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GENBANK/AF128450
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GENBANK/AF128451
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GENBANK/AF128452