Isolation of T-cell antigens by retrovirus-mediated expression cloning

Methods Mol Biol. 2000:134:143-52. doi: 10.1385/1-59259-682-7:143.

Authors

T Kitamura¹, Y Morikawa

Affiliation

¹ Department of Hematopoietic Factors, University of Tokyo, Japan.

PMID: 10730255
DOI: 10.1385/1-59259-682-7:143

No abstract available

MeSH terms

3T3 Cells
Animals
Cloning, Molecular / methods
DNA, Complementary
Electroporation / methods
Gene Library
Genetic Vectors
Mice
Polymerase Chain Reaction
Receptors, Antigen, T-Cell / genetics*
Receptors, Antigen, T-Cell / isolation & purification*
Recombinant Proteins / isolation & purification
Retroviridae
Reverse Transcriptase Polymerase Chain Reaction
Transfection / methods

Substances

DNA, Complementary
Receptors, Antigen, T-Cell
Recombinant Proteins