Porcine postweaning multisystemic wasting syndrome in Korean pig: detection of porcine circovirus 2 infection by immunohistochemistry and polymerase chain reaction

J Vet Diagn Invest. 2000 Mar;12(2):151-3. doi: 10.1177/104063870001200209.

Abstract

This report describes the first diagnosis of porcine circovirus (PCV) infection in weaned pigs with postweaning multisystemic wasting syndrome in Korea by immunohistochemistry and polymerase chain reaction. The most unique lesions were multifocal granulomatous inflammation affecting lymph nodes, liver, and spleen, characterized by infiltrates of epithelioid macrophages and multinucleated giant cells. Circoviral antigen was detected in formalin-fixed sections and was usually present in large, round, dendritic cells in the white pulp of spleen and remnants of follicles in lymph nodes. Lymphoid follicles in the tonsils also contained PCV antigen. A 530-bp DNA fragment of circovirus was successfully amplified from all tested lymph nodes, liver, and spleen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Circoviridae Infections / diagnosis
  • Circoviridae Infections / veterinary*
  • Circoviridae Infections / virology
  • Circovirus / immunology*
  • DNA, Viral / analysis
  • Disease Outbreaks
  • Immunohistochemistry
  • Korea / epidemiology
  • Polymerase Chain Reaction / veterinary
  • Swine
  • Swine Diseases / diagnosis
  • Swine Diseases / immunology
  • Swine Diseases / virology*
  • Wasting Syndrome / diagnosis
  • Wasting Syndrome / veterinary*
  • Wasting Syndrome / virology

Substances

  • DNA, Viral