Abstract
Correct positioning of the mitotic spindle is critical for cell division and development. Spindle positioning involves a search-and-capture mechanism whereby dynamic microtubules find and then interact with specific sites on the submembrane cortex. Genetic, biochemical, and imaging experiments suggest a mechanism for cortical-microtubule capture. Bim1p, located at microtubule distal ends, bound Kar9p, a protein associated with the daughter cell cortex. Bim1p is the yeast ortholog of human EB1, a binding partner for the adenomatous polyposis coli tumor suppressor. EB1 family proteins may have a general role in linking the microtubule cytoskeleton to cortical polarity determinants.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenomatous Polyposis Coli Protein
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Binding Sites
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Cell Cycle
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Cytoskeletal Proteins / metabolism
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G1 Phase
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Microtubule Proteins / genetics
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Microtubule Proteins / metabolism*
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Microtubule-Associated Proteins / metabolism
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Microtubules / metabolism*
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Protein Binding
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins*
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Spindle Apparatus / physiology*
Substances
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Adenomatous Polyposis Coli Protein
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BIM1 protein, S cerevisiae
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Cell Cycle Proteins
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Cytoskeletal Proteins
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EB1 microtubule binding proteins
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KAR9 protein, S cerevisiae
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Microtubule Proteins
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Microtubule-Associated Proteins
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Nuclear Proteins
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins