Abstract
Endogenous lectins are proteins/glycoproteins which selectively recognize distinct saccharide ligands and are different from immunoglobulins and carbohydrate-utilizing enzymes. Expression of these molecules can be detected immunohistochemically using nonblocking monoclonal antibodies (A1D6: anti-galectin-3, MR-15-2-2: anti-175 kD mannose receptor). Alternatively, biotinylated (neo)glycoconjugates which are recognized by a studied lectin can be employed as convenient probes to demonstrate specific binding of sugar epitopes by carbohydrate recognition domains (CRD) of endogenous lectins. In this study, we describe a new procedure for immunocytochemical visualization of the expression of endogenous lectins and glycochemical visualization of the reactivity of carbohydrate recognition domain(s), performed simultaneously at the single-cell level.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibodies, Monoclonal / immunology
-
Antigens, Differentiation / analysis
-
Antigens, Differentiation / immunology
-
Biotinylation
-
Epitopes / immunology
-
Galectin 3
-
Giant Cells / chemistry*
-
Glycoconjugates / metabolism
-
Humans
-
Immunoenzyme Techniques*
-
Lectins / analysis*
-
Lectins / immunology
-
Lectins, C-Type*
-
Ligands
-
Macrophages, Alveolar / chemistry*
-
Mannose Receptor
-
Mannose-Binding Lectins*
-
Oligosaccharides / immunology
-
Oligosaccharides / metabolism
-
Rats
-
Receptors, Cell Surface / analysis
-
Receptors, Cell Surface / immunology
-
Receptors, Mitogen / analysis*
-
Receptors, Mitogen / immunology
Substances
-
Antibodies, Monoclonal
-
Antigens, Differentiation
-
Epitopes
-
Galectin 3
-
Glycoconjugates
-
Lectins
-
Lectins, C-Type
-
Ligands
-
Mannose Receptor
-
Mannose-Binding Lectins
-
Oligosaccharides
-
Receptors, Cell Surface
-
Receptors, Mitogen