Abstract
Ticks play an important role in human and veterinary medicine, in particular due to their ability to transmit a wide spectrum of pathogenic micro-organisms of protozoal, rickettsial, bacterial and viral origin. Pathogens in ticks can be identified by conventional methods such as indirect immunofluorescence, isolation in cell culture or by using histological staining techniques. However, the advent of the polymerase chain reaction (PCR) has resulted in tremendous improvements in the specific and sensitive detection of pathogen DNA in ticks. In this paper, literature on DNA extraction methods, PCR protocols, primers and probes, which are in use for the successful detection and identification of pathogens in ticks, are critically reviewed. Some recommendations are also given towards the end of this review.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Anaplasma / genetics
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Anaplasma / isolation & purification
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Animals
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Arachnid Vectors / microbiology*
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Arachnid Vectors / parasitology
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Arachnid Vectors / virology
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Babesia / genetics
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Babesia / isolation & purification
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Borrelia / genetics
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Borrelia / isolation & purification
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DNA Primers / chemistry
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DNA, Bacterial / chemistry
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DNA, Bacterial / isolation & purification*
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DNA, Protozoan / chemistry
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DNA, Protozoan / isolation & purification*
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DNA, Viral / chemistry
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DNA, Viral / isolation & purification*
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Ehrlichia / genetics
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Ehrlichia / isolation & purification
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Ehrlichia ruminantium / genetics
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Ehrlichia ruminantium / isolation & purification
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Encephalitis Viruses, Tick-Borne / genetics
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Encephalitis Viruses, Tick-Borne / isolation & purification
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Nairovirus / genetics
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Nairovirus / isolation & purification
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Polymerase Chain Reaction / methods
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Polymerase Chain Reaction / veterinary
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Theileria / genetics
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Theileria / isolation & purification
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Tick-Borne Diseases / transmission
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Tick-Borne Diseases / veterinary*
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Ticks / microbiology*
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Ticks / parasitology
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Ticks / virology
Substances
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DNA Primers
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DNA, Bacterial
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DNA, Protozoan
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DNA, Viral