Abstract
To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, we transferred HGF gene into liver of the Solt-Farber rat model. Male Fisher 344 rats were infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and diminished thereafter. The total and proliferating cell nuclear antigen-positive hepatic oval cells were significantly elevated in HGF-transferred rats, in which stem cell factor and c-kit mRNA increased at each time point. Our results suggest that in vivo transfer of the HGF gene into liver accelerates proliferation of hepatic oval cells in the Solt-Farber model in rats.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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2-Acetylaminofluorene / toxicity*
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Animals
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Cell Division / drug effects
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Disease Models, Animal*
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Gene Transfer Techniques
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Hepatectomy*
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Hepatocyte Growth Factor / genetics
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Hepatocyte Growth Factor / physiology*
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Immunohistochemistry
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Liver / cytology*
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Liver / drug effects*
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Liver / metabolism
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Liver / surgery
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Male
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Proliferating Cell Nuclear Antigen / analysis
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Proto-Oncogene Proteins c-kit / genetics
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Proto-Oncogene Proteins c-met / genetics
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Rats
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Rats, Inbred F344
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Stem Cell Factor / genetics
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Time Factors
Substances
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Proliferating Cell Nuclear Antigen
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RNA, Messenger
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Stem Cell Factor
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Hepatocyte Growth Factor
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2-Acetylaminofluorene
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Proto-Oncogene Proteins c-kit
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Proto-Oncogene Proteins c-met