High prevalence of renal transplant recipients infected with more than one cytomegalovirus glycoprotein B genotype

J Med Virol. 2000 May;61(1):138-42.

Abstract

A prospective analysis of cytomegalovirus (CMV) glycoprotein B (gB) genotypes was conducted on 34 renal transplant recipients using peripheral blood leukocytes (PBLs) and urine specimens. The CMV gB genotypes were analyzed by polymerase chain reaction (PCR) followed by enzyme digestion. PBLs and urine samples showed almost equal proportions of the 4 known gB genotypes, as well as equal proportions of gB genotype mixtures. The gB genotypes 1, 2 and 3 were equally distributed in the patients. Twenty-four (70.6%) patients had more than one gB genotype during follow-up. There was no association of gB genotypes with the development of symptomatic CMV infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytomegalovirus / genetics*
  • Cytomegalovirus Infections / epidemiology*
  • Cytomegalovirus Infections / virology
  • DNA, Viral / analysis
  • Female
  • Genotype
  • Humans
  • Kidney Transplantation / adverse effects*
  • Male
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Prevalence
  • Prospective Studies
  • Sensitivity and Specificity
  • Viral Envelope Proteins / blood
  • Viral Envelope Proteins / genetics*
  • Viral Envelope Proteins / urine

Substances

  • DNA, Viral
  • Viral Envelope Proteins
  • glycoprotein B, Simplexvirus