The Na+-Ca2+ exchange inhibitor KB-R7943 inhibits high K+-induced increases in intracellular Ca2+ concentration and [3H]noradrenaline release in the human neuroblastoma SH-SY5Y

H Nakamura; Y Kawasaki; N Arakawa; M Saeki; S Maeda; Y Koyama; A Baba; T Matsuda

doi:10.1023/a:1007597105714

The Na+-Ca2+ exchange inhibitor KB-R7943 inhibits high K+-induced increases in intracellular Ca2+ concentration and [3H]noradrenaline release in the human neuroblastoma SH-SY5Y

Neurochem Res. 2000 Mar;25(3):385-7. doi: 10.1023/a:1007597105714.

Authors

H Nakamura¹, Y Kawasaki, N Arakawa, M Saeki, S Maeda, Y Koyama, A Baba, T Matsuda

Affiliation

¹ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan.

PMID: 10761983
DOI: 10.1023/a:1007597105714

Abstract

The effects of the Na+-Ca2+ exchange inhibitor 2-[2-[4-(4-nitrobenzyloxy)phenyl]ethyl]isothiourea methanesulfonate (KB-R7943) on depolarization-induced Ca2+ signal and [3H]noradrenaline release were examined in SH-SY5Y cells. KB-R7943 at 10 microM significantly inhibited high K+-induced increase in intracellular Ca2+ concentration. KB-R7943 also inhibited high K+-evoked release of [3H]noradrenaline from the cells. These findings suggest that the Na+-Ca2+ exchanger in the reverse mode is involved at least partly in depolarization-induced transmitter release.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calcium / metabolism*
Enzyme Inhibitors / pharmacology*
Humans
Intracellular Fluid / enzymology
Intracellular Fluid / metabolism
Neuroblastoma
Norepinephrine / metabolism*
Potassium / pharmacology*
Sodium-Calcium Exchanger / antagonists & inhibitors*
Thiourea / analogs & derivatives*
Thiourea / pharmacology
Tritium
Tumor Cells, Cultured

Substances

2-(2-(4-(4-nitrobenzyloxy)phenyl)ethyl)isothiourea methanesulfonate
Enzyme Inhibitors
Sodium-Calcium Exchanger
Tritium
Thiourea
Potassium
Calcium
Norepinephrine