Site-specific serine incorporation by Lif and Epr into positions 3 and 5 of the Staphylococcal peptidoglycan interpeptide bridge

J Bacteriol. 2000 May;182(9):2635-8. doi: 10.1128/JB.182.9.2635-2638.2000.

Abstract

The FemAB-like factors Lif and Epr confer resistance to glycylglycine endopeptidases lysostaphin and Ale-1, respectively, by incorporating serine residues into the staphylococcal peptidoglycan interpeptide bridges specifically at positions 3 and 5. This required the presence of FemA and/or FemB, in contrast to earlier postulations.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Drug Resistance, Microbial*
  • Mutagenesis, Site-Directed
  • Peptides / metabolism
  • Peptidoglycan / metabolism*
  • Serine / genetics
  • Serine / metabolism*
  • Staphylococcus / genetics
  • Staphylococcus / metabolism

Substances

  • Bacterial Proteins
  • Epr protein, Staphylococcus capitis
  • FemA protein, Bacteria
  • FemB protein, Staphylococcus epidermidis
  • Peptides
  • Peptidoglycan
  • Serine