FHA domain boundaries of the dun1p and rad53p cell cycle checkpoint kinases

FEBS Lett. 2000 Apr 14;471(2-3):141-6. doi: 10.1016/s0014-5793(00)01392-2.

Abstract

Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130-140 residues, and are thus considerably larger than previously predicted by sequence comparisons (55-75 residues). In vivo, expression of the proteolytically defined Dun1p FHA domain, but not a fragment containing only the predicted domain boundaries, inhibited the transcriptional induction of repair genes following replication blocks. This indicates that the non-catalytic FHA domain plays an important role in the transcriptional function of the Dun1p protein kinase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Catalytic Domain* / genetics
  • Catalytic Domain* / physiology
  • Cell Cycle Proteins*
  • Checkpoint Kinase 2
  • Chymotrypsin / metabolism
  • DNA Replication / drug effects
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal
  • Genes, Fungal / genetics
  • Hydroxyurea / pharmacology
  • Molecular Sequence Data
  • Molecular Weight
  • Peptide Fragments / chemistry
  • Peptide Fragments / genetics
  • Peptide Fragments / metabolism
  • Protein Kinases / chemistry*
  • Protein Kinases / genetics
  • Protein Kinases / metabolism*
  • Protein Serine-Threonine Kinases*
  • Protein Structure, Tertiary
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Serine Endopeptidases / metabolism
  • Transcription, Genetic / genetics

Substances

  • Cell Cycle Proteins
  • Fungal Proteins
  • Peptide Fragments
  • RNA, Messenger
  • Saccharomyces cerevisiae Proteins
  • Protein Kinases
  • DUN1 protein, S cerevisiae
  • Checkpoint Kinase 2
  • Protein Serine-Threonine Kinases
  • RAD53 protein, S cerevisiae
  • Serine Endopeptidases
  • Chymotrypsin
  • glutamyl endopeptidase
  • Hydroxyurea