Abstract
Expression of CD40 ligand (CD40L) correlated directly with Mycobacterium tuberculosis-stimulated gamma interferon (IFN-gamma) production by peripheral blood mononuclear cells (PBMC) from tuberculosis patients and healthy tuberculin reactors. The CD40L agonist increased M. tuberculosis-induced IFN-gamma production by PBMC, and anti-CD40 or anti-CD40L antibodies reduced IFN-gamma production. CD40L expression on PBMC was reduced by exposure to B cells and to soluble factors from M. tuberculosis-infected monocytes. These findings suggest that CD40L dysregulation contributes to reduced IFN-gamma production in human tuberculosis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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B-Lymphocytes / immunology
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CD40 Antigens / biosynthesis
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CD40 Ligand
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Cells, Cultured
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Humans
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Interferon-gamma / biosynthesis*
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Leukocytes, Mononuclear
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Lymphocyte Depletion
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Membrane Glycoproteins / biosynthesis*
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Mycobacterium tuberculosis / immunology
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Receptors, Interleukin / biosynthesis
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Receptors, Interleukin-12
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Tuberculin / immunology
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Tuberculosis / blood
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Tuberculosis / drug therapy
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Tuberculosis / immunology*
Substances
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CD40 Antigens
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Membrane Glycoproteins
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Receptors, Interleukin
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Receptors, Interleukin-12
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Tuberculin
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CD40 Ligand
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Interferon-gamma