Abstract
The protease, reverse transcriptase (RT), and envelope (env) genes of human immunodeficiency virus type 1 (HIV-1) clinical isolates from 13 immigrants (mainly of African origin) living in Spain were examined. Phylogenetic analyses were performed, taking as reference a panel of 25 HIV-1 sequences representing various subtypes. A discrepant topology was recognized in comparing the protease, RT, and/or env phylogenetic trees in 10 isolates, in which sequences clustering in 2 or 3 different HIV-1 subtypes were found. In eight of these strains, the discrepant region was env with respect to concordant pol genes. Five of nine patients harboring subtype G sequences at the protease and RT genes showed discrepant env sequences, being subtype A (three) or B (two). In addition, one recombinant H/A strain, one recombinant H/B isolate, and a triple recombinant, A/D/C, variant were found. This work represents the first phylogenetic characterization of HIV-1 recombinant clinical isolates in Spain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Africa / ethnology
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Base Sequence
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Endopeptidases / genetics
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Female
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Genes, Viral / genetics
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Genes, env / genetics
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Genetic Variation / genetics
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HIV Infections / epidemiology*
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HIV-1 / enzymology
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HIV-1 / genetics*
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Humans
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Male
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Middle Aged
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Molecular Sequence Data
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Phylogeny
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Portugal / ethnology
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RNA-Directed DNA Polymerase / genetics
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Spain / epidemiology
Substances
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RNA-Directed DNA Polymerase
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Endopeptidases
Associated data
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GENBANK/AF125281
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GENBANK/AF125283
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GENBANK/AF125285
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GENBANK/AF125288
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GENBANK/AF125289
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GENBANK/AF125290
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GENBANK/AF125291
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GENBANK/AF125292
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GENBANK/AF125295
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GENBANK/AF125296
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GENBANK/AF125297
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GENBANK/AF125298
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GENBANK/AF125299
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GENBANK/AF125300
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GENBANK/AF125301
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GENBANK/AF125302
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GENBANK/AF188335
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GENBANK/AF188336
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GENBANK/AF188337
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GENBANK/AF188338
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GENBANK/AF188339
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GENBANK/AF188340
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GENBANK/AF188341
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GENBANK/AF188342
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GENBANK/AF188343
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GENBANK/AF188344
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GENBANK/AF188345
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GENBANK/AF188346
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GENBANK/AF188347
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GENBANK/AF188348