Abstract
The transportability of cytosine-containing nucleosides by recombinant hCNT1 was investigated in transfected mammalian cells. Apparent K(m) values for hCNT1-mediated transport of uridine, cytidine and deoxycytidine were, respectively, 59, 140 and 150 microM. Uridine transport was inhibited 89, 32 and 11%, respectively, by 500 microM gemcitabine, cytarabine and lamivudine, demonstrating that, unlike gemcitabine (a high-affinity hCNT1 permeant), cytarabine and lamivudine are poor hCNT1 permeants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antimetabolites, Antineoplastic / pharmacology
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Antiviral Agents / pharmacology
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Biological Transport
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COS Cells
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Carrier Proteins / metabolism*
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Cell Division / drug effects
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Cell Line
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Cytarabine / pharmacology
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Cytidine / metabolism*
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Deoxycytidine / analogs & derivatives
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Deoxycytidine / metabolism*
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Deoxycytidine / pharmacology
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Gemcitabine
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Haplorhini
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HeLa Cells
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Humans
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Immunoblotting
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Lamivudine / pharmacology
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Membrane Transport Proteins*
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Recombinant Proteins / metabolism
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Substrate Specificity
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Transfection
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Uridine / metabolism*
Substances
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Antimetabolites, Antineoplastic
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Antiviral Agents
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Carrier Proteins
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Membrane Transport Proteins
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Recombinant Proteins
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cif nucleoside transporter
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Cytarabine
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Deoxycytidine
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Lamivudine
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Cytidine
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Uridine
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Gemcitabine