FISH-microdissection (FISH-MD) analysis of complex chromosome rearrangements

Cytogenet Cell Genet. 2000;88(1-2):114-8. doi: 10.1159/000015502.

Abstract

We combined the techniques of fluorescence in situ hybridization (FISH) and chromosomal microdissection in one experiment (FISH-MD). This novel method permits rapid identification of the composition, origin, and breakpoints of rearranged chromosomes. Rearranged chromosomes are first identified by multicolor-FISH, then the fluorophore-labeled derivative chromosomes are directly isolated by microdissection and reverse painted to identify the breakpoints.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Aberrations / genetics*
  • Chromosome Banding / methods
  • Chromosome Breakage / genetics
  • Chromosome Painting / methods*
  • Color
  • Female
  • Fluorescent Dyes / metabolism
  • Humans
  • Indoles / metabolism
  • Karyotyping / methods
  • Light
  • Lymphocytes
  • Mixed Tumor, Mullerian / genetics
  • Mutagenesis, Insertional / genetics
  • Physical Chromosome Mapping / methods*
  • Polymerase Chain Reaction
  • Translocation, Genetic / genetics

Substances

  • Fluorescent Dyes
  • Indoles
  • DAPI