In vivo antibody response and in vitro CTL activation induced by selected measles vaccine candidates, prepared with purified Quil A components

K J Stittelaar; J Boes; G F Kersten; A Spiekstra; P G Mulder; P de Vries; P J Roholl; K Dalsgaard; G van den Dobbelsteen; L van Alphen; A D Osterhaus

doi:10.1016/s0264-410x(00)00026-8

In vivo antibody response and in vitro CTL activation induced by selected measles vaccine candidates, prepared with purified Quil A components

Vaccine. 2000 May 22;18(23):2482-93. doi: 10.1016/s0264-410x(00)00026-8.

Authors

K J Stittelaar¹, J Boes, G F Kersten, A Spiekstra, P G Mulder, P de Vries, P J Roholl, K Dalsgaard, G van den Dobbelsteen, L van Alphen, A D Osterhaus

Affiliation

¹ Laboratory for Vaccine Research, Institute of Public Health and the Environment, 3720 BA, Bilthoven, The Netherlands.

PMID: 10775782
DOI: 10.1016/s0264-410x(00)00026-8

Abstract

Semipurified Quil A and purified Quil A were used to prepare well-characterized subunit vaccine candidates against measles. Variation in the relative amounts of the measles virus (MV) fusion (F) protein, Quil A-components and lipids did not influence induction of antibody responses in mice, but had a pronounced effect on the capacity to induce cytotoxic T cell (CTL) activity of a CD8(+) MV F-protein specific human T cell clone in vitro. A characteristic MV iscom preparation based on the combined use of HPLC-purified Quil A-components QA-3 and QA-22 (QA-3/22) efficiently induced CTL activity in vitro. Comparable results were obtained by mixing beta-propiolactone inactivated MV with iscom-matrix QA-3/22 or free QA-22. On the basis of the data presented it was concluded that these three preparations are interesting MV vaccine candidates for further evaluation in pre-clinical experiments in a primate model.

Publication types

Comparative Study

MeSH terms

Adjuvants, Immunologic / chemistry*
Adjuvants, Immunologic / isolation & purification
Animals
Antibodies, Viral / biosynthesis*
Cells, Cultured
Chlorocebus aethiops
Chromatography, High Pressure Liquid
Cytotoxicity, Immunologic
Hemagglutinins, Viral / immunology*
Humans
ISCOMs / chemistry
ISCOMs / immunology*
Lymphocyte Activation*
Measles Vaccine / chemistry
Measles Vaccine / immunology*
Measles virus / drug effects
Measles virus / immunology*
Mice
Mice, Inbred BALB C
Microscopy, Electron
Propiolactone / pharmacology
Quillaja Saponins
Saponins / chemistry
Saponins / immunology*
T-Lymphocytes, Cytotoxic / immunology*
Vaccination
Vaccines, Attenuated / immunology
Vero Cells
Viral Fusion Proteins / immunology*

Substances

Adjuvants, Immunologic
Antibodies, Viral
Hemagglutinins, Viral
ISCOMs
Measles Vaccine
Quillaja Saponins
Saponins
Vaccines, Attenuated
Viral Fusion Proteins
hemagglutinin protein G, measles virus
Quil A
Propiolactone