Abstract
It has been shown that macrophage migration inhibitory factor (MIF) modulates not only macrophage functions, but also T cell functions. However, detailed analysis of the MIF function on responses of various T cell subpopulations remained to be elucidated. In this report, using a neutralizing anti-MIF monoclonal antibody (mAb) we examined MIF functions on various T cell lineages. It was shown that anti-MIF mAb inhibited antigen-specific responses of both IFN-gamma producing and IL-4 producing T cells. The inhibition appeared to be related to blockade of the signal pathway via T cell receptor (TCR) but not that via IL-2 receptor (IL-2R). However, the anti-MIF mAb showed no inhibitory effect on NK-T cell responses stimulated through TCR. These results suggest that MIF is involved in the signal pathway via TCR in mainstream T cells but not in NK-T cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / immunology
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Antibody Specificity / immunology
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Antigens / immunology
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Antigens, Surface
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Cell Division
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Cell Line
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Humans
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Killer Cells, Natural / cytology
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Killer Cells, Natural / metabolism
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Lectins, C-Type
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Lymphocyte Activation
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Macrophage Migration-Inhibitory Factors / biosynthesis
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Macrophage Migration-Inhibitory Factors / immunology*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Molecular Sequence Data
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NK Cell Lectin-Like Receptor Subfamily B
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Proteins / immunology
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Receptors, Antigen, T-Cell / immunology
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Receptors, Interleukin-2 / immunology
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Signal Transduction / immunology*
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T-Lymphocyte Subsets / immunology*
Substances
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Antibodies, Monoclonal
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Antigens
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Antigens, Surface
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KLRB1 protein, human
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Lectins, C-Type
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Macrophage Migration-Inhibitory Factors
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NK Cell Lectin-Like Receptor Subfamily B
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Proteins
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Receptors, Antigen, T-Cell
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Receptors, Interleukin-2