Upregulation of bradykinin B1-receptor expression after myocardial infarction

Br J Pharmacol. 2000 Apr;129(8):1537-8. doi: 10.1038/sj.bjp.0703239.

Abstract

To determine the influence of the myocardial infarction (MI) on bradykinin B1-receptor (B1R) regulation, we studied its expression in the left ventricle (LV) after MI. Rats were submitted to a permanent occlusion of the left coronary artery. Six hours, 24 h and 6 days after MI or after sham operation, left ventricular pressure (LVP) and dP/dt(max) were measured. LV-total RNA was extracted and B1R expression was analysed by a RNase-protection assay (each group n = 6). LVP and dP/dt(max) were impaired at all time points after MI. Basal B1R expression was not detectable in controls. Six hours after MI, the B1R expression was upregulated and reached a maximum 24 h after MI (4 fold vs. 6 h). Six days post-MI, B1R expression returned to levels found 6 h after MI. These data are the first demonstration for an induced myocardial B1R expression in an in vivo model of MI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Hemodynamics
  • Male
  • Myocardial Infarction / metabolism*
  • Myocardial Infarction / physiopathology
  • Rats
  • Rats, Sprague-Dawley
  • Receptor, Bradykinin B1
  • Receptors, Bradykinin / biosynthesis*
  • Receptors, Bradykinin / metabolism
  • Up-Regulation
  • Ventricular Function, Left

Substances

  • Receptor, Bradykinin B1
  • Receptors, Bradykinin