Molecular cloning of the CRM1 gene from Candida albicans

Yeast. 2000 Apr;16(6):531-8. doi: 10.1002/(SICI)1097-0061(200004)16:6<531::AID-YEA543>3.0.CO;2-J.

Abstract

In a screen for Candida albicans genes capable of supressing a ste20Delta mutation in Saccharomyces cerevisiae, a homologue of the exportin-encoding gene CRM1 was isolated. The CaCRM1 gene codes for a protein of 1079 amino acids with a predicted molecular weight of 124 029 and isoelectric point of 5.04. Crm1p from C. albicans displays significant amino acid sequence homology with Crm1p from Saccharomyces cerevisiae (65% identity, 74% similarity), Schizosaccharomyces pombe (55% identity, 66% similarity), Caenorhabditis elegans (45% identity, 57% similarity), and Homo sapiens (48% identity, 59% similarity). Interestingly, CaCRM1 encodes a threonine rather than a cysteine at position 533 in the conserved central region, suggesting that CaCrm1p is leptomycin B-insensitive, like S. cerevisiae Crm1p. CaCRM1 on a high copy vector can complement a thermosensitive allele of CRM1 (xpo1-1) in S. cerevisiae, showing that CaCrm1p and S. cerevisiae Crm1p are functionally conserved. Southern blot analysis suggests that CaCRM1 is present at a single locus within the C. albicans genome. The nucleotide sequence of the CaCRM1 gene has been deposited at GenBank under Accession No. AF178855.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blotting, Southern
  • Candida albicans / genetics*
  • Carrier Proteins / genetics*
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • Exportin 1 Protein
  • Fungal Proteins / genetics*
  • Genes, Fungal / genetics
  • Genetic Complementation Test
  • Karyopherins*
  • Molecular Sequence Data
  • Receptors, Cytoplasmic and Nuclear*
  • Saccharomyces cerevisiae / genetics
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • Carrier Proteins
  • DNA, Fungal
  • Fungal Proteins
  • Karyopherins
  • Receptors, Cytoplasmic and Nuclear

Associated data

  • GENBANK/AF178855