Interactions of inflammatory mediators and low pH not influenced by capsazepine in rat cutaneous nociceptors

Neuroreport. 2000 Apr 7;11(5):973-6. doi: 10.1097/00001756-200004070-00015.

Abstract

The rat skin-saphenous nerve preparation was used to record from mechano-heat sensitive C-fibers whose receptive fields were superfused with various solutions of low pH and of bradykinin, serotonin and prostaglandin E2. Only synchronous application of protons and mediators resulted in a significant nearly three-fold augmentation of the nociceptive pH response, and capsazepine (10(-5) M) did not block this short-lived enhancement. Thus, it does not seem to involve the capsaicin receptor (VRI) which is in contrast to a previous finding from cultured sensory neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bradykinin / pharmacology
  • Capsaicin / analogs & derivatives*
  • Capsaicin / pharmacology
  • Dinoprostone / pharmacology
  • Drug Interactions / physiology*
  • Ganglia, Spinal / drug effects
  • Ganglia, Spinal / metabolism
  • Hydrogen-Ion Concentration*
  • Inflammation / chemically induced*
  • Inflammation / pathology
  • Inflammation / physiopathology*
  • Inflammation Mediators / metabolism*
  • Inflammation Mediators / pharmacology*
  • Male
  • Nerve Fibers / drug effects
  • Nerve Fibers / metabolism
  • Nociceptors / drug effects*
  • Nociceptors / metabolism*
  • Oxytocics / pharmacology
  • Protons / adverse effects
  • Rats
  • Rats, Wistar
  • Serotonin / pharmacology
  • Signal Transduction / drug effects
  • Signal Transduction / physiology
  • Skin / drug effects*
  • Skin / innervation*
  • Skin / physiopathology

Substances

  • Inflammation Mediators
  • Oxytocics
  • Protons
  • Serotonin
  • Dinoprostone
  • capsazepine
  • Capsaicin
  • Bradykinin