Abstract
Proteins that bind and hydrolyze ATP are frequently involved in the early steps of DNA replication. Recent studies of Saccharomyces cerevisiae suggest that two members of the AAA+ ATPase family--the origin recognition complex and Cdc6p--have separable roles for ATP binding and ATP hydrolysis during eukaryotic DNA replication. Intriguingly, the proposed regulation of these eukaryotic replication proteins by ATP has functional similarities to the ATP-dependent control of the DnaA and DnaC initiation factors from Escherichia coli. Comparison of the ATP regulation of these factors suggests that ATP binding and hydrolysis acts as a molecular switch that couples key events during initiation of replication. This switch results in a significant change in protein function.
MeSH terms
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Adenosine Triphosphatases / metabolism*
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Adenosine Triphosphate / metabolism
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Bacterial Proteins / metabolism
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism
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DNA Helicases / metabolism
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DNA Replication / physiology*
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DNA-Binding Proteins / metabolism
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Escherichia coli / metabolism
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Escherichia coli Proteins*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Mutation
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
Substances
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Bacterial Proteins
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CDC6 protein, S cerevisiae
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Cell Cycle Proteins
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DNA-Binding Proteins
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DnaA protein, Bacteria
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DnaC protein, E coli
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Escherichia coli Proteins
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Fungal Proteins
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Saccharomyces cerevisiae Proteins
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Adenosine Triphosphate
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Adenosine Triphosphatases
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DNA Helicases