Abstract
Cytokines play an important role in modulating inflammatory responses and, as a result, airway tone. IL-10 is a regulatory cytokine that has been suggested for treatment of asthma because of its immunosuppressive and anti-inflammatory properties. In contrast to these suggestions, we demonstrate in a model of allergic sensitization that mice deficient in IL-10 (IL-10-/-) develop a pulmonary inflammatory response but fail to exhibit airway hyperresponsiveness in both in vitro and in vivo assessments of lung function. Reconstitution of these deficient mice with the IL-10 gene fully restores development of airway hyperresponsiveness comparable to control mice. These results identify an important role of IL-10, downstream of the inflammatory cascade, in regulating the tone of the airways after allergic sensitization and challenge.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aerosols
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Animals
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Blood Proteins / analysis
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Bronchial Hyperreactivity / physiopathology*
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Bronchial Provocation Tests
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Bronchoalveolar Lavage Fluid / chemistry
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Cytokines / analysis
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Electric Stimulation
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Eosinophil Granule Proteins
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Eosinophil Peroxidase
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Eosinophilia / etiology
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Eosinophilia / physiopathology
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Female
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Genetic Complementation Test
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Genetic Therapy
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Immunization
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Inflammation / physiopathology
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Interleukin-10 / deficiency
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Interleukin-10 / genetics
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Interleukin-10 / physiology*
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Leukotrienes / analysis
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Lung / chemistry
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Lung / pathology
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Male
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Methacholine Chloride / administration & dosage
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Muscle, Smooth / physiopathology
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Ovalbumin / administration & dosage
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Ovalbumin / immunology
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Peroxidases / analysis
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Respiratory Hypersensitivity / physiopathology*
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Ribonucleases*
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Specific Pathogen-Free Organisms
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Trachea / physiopathology
Substances
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Aerosols
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Blood Proteins
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Cytokines
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Eosinophil Granule Proteins
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Leukotrienes
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Methacholine Chloride
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Interleukin-10
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Ovalbumin
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Eosinophil Peroxidase
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Peroxidases
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Ribonucleases