TCR-Vbeta repertoire analysis with RT-PCR was useful for the early detection of pulmonary relapsed T-cell lymphoma after autologous peripheral blood stem cell transplantation

Am J Hematol. 2000 Jun;64(2):124-7. doi: 10.1002/(sici)1096-8652(200006)64:2<124::aid-ajh10>3.0.co;2-6.

Abstract

Pulmonary recurrence of malignant lymphoma is a rare event after stem cell transplantation. We report here a 45-year-old male who was successfully diagnosed with relapsed pulmonary T-cell lymphoma using an RT-PCR method. Clonal expansion of T cells expressing identical TCR V-D-J junction size (Vbeta5-Jbeta1.5) was demonstrated in lymphocyte groups obtained from both bronchoalveolar lavage fluid at relapse, and paraffin embedded lymph node samples resected when he was first diagnosed with angioimmunoblastic T-cell lymphoma. This method provided evidence to diagnose relapsed pulmonary angioimmunoblastic T-cell lymphoma in its early phase.

Publication types

  • Case Reports

MeSH terms

  • Bronchoalveolar Lavage Fluid / cytology
  • Hematopoietic Stem Cell Transplantation*
  • Humans
  • Lung Neoplasms / diagnosis*
  • Lung Neoplasms / metabolism*
  • Lung Neoplasms / surgery
  • Lymph Nodes / metabolism
  • Lymph Nodes / pathology
  • Lymphocytes / metabolism
  • Lymphoma, T-Cell / diagnosis*
  • Lymphoma, T-Cell / metabolism*
  • Lymphoma, T-Cell / surgery
  • Male
  • Middle Aged
  • Neoplasm Recurrence, Local / diagnosis*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / diagnosis
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / metabolism
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / surgery
  • Receptors, Antigen, T-Cell, alpha-beta / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Receptors, Antigen, T-Cell, alpha-beta