Abstract
Parkinson's disease (PD) is a leading candidate for neurological gene therapy, given our increasing knowledge of the functional anatomy of the striatonigral system and the localized nature of the affected cell populations. Here we report that stereotactic introduction of a human tyrosine hydroxylase (TH-2) gene using multi-site partitioned doses resulted in behavioral recovery in 6-OHDA-lesioned rats, with transient 100% recovery observed in some animals. We also show correlation between numbers of TH-immunoreactive cells and loss of apomorphine induced rotation, with a near-linear relationship between TH expression and phenotypic recovery. Furthermore, the data suggest that only a fraction of striatal cells need to be transduced in order to exert phenotypic effects, and therefore TH partitioned gene transfer may have clinical potential in PD.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae / genetics
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Adrenergic Agents
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Analysis of Variance
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Animals
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Apomorphine
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Behavior, Animal
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Cell Count
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Corpus Striatum / enzymology
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Corpus Striatum / pathology
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Disease Models, Animal
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Gene Transfer Techniques*
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Genetic Therapy*
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Genetic Vectors / administration & dosage
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Genetic Vectors / genetics
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Humans
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Male
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Motor Activity
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Oxidopamine
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Parkinson Disease, Secondary / chemically induced
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Parkinson Disease, Secondary / diagnosis
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Parkinson Disease, Secondary / genetics
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Parkinson Disease, Secondary / therapy*
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Phenotype
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Rats
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Rats, Inbred F344
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Recovery of Function
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Treatment Outcome
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Tyrosine 3-Monooxygenase / administration & dosage*
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Tyrosine 3-Monooxygenase / genetics*
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Tyrosine 3-Monooxygenase / metabolism
Substances
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Adrenergic Agents
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Oxidopamine
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Tyrosine 3-Monooxygenase
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Apomorphine