3D EXAFS refinement of the Cu site of azurin sheds light on the nature of structural change at the metal centre in an oxidation-reduction process: an integrated approach combining EXAFS and crystallography

K C Cheung; R W Strange; S S Hasnain

doi:10.1107/s0907444900003310

3D EXAFS refinement of the Cu site of azurin sheds light on the nature of structural change at the metal centre in an oxidation-reduction process: an integrated approach combining EXAFS and crystallography

Acta Crystallogr D Biol Crystallogr. 2000 Jun;56(Pt 6):697-704. doi: 10.1107/s0907444900003310.

Authors

K C Cheung¹, R W Strange, S S Hasnain

Affiliation

¹ Faculty of Applied Sciences, De Montfort University, Leicester. England.

PMID: 10818346
DOI: 10.1107/s0907444900003310

Abstract

Three-dimensional information is obtained for the Cu site in azurin at very high resolution by combining high-resolution crystallographic structures and EXAFS data for the oxidized and reduced form of the protein. This combined approach has allowed us to define the subtle structural changes (<0.1 A) which take place at the Cu site during a single-electron redox process.

MeSH terms

Alcaligenes
Azurin / chemistry*
Azurin / metabolism
Computer Simulation
Copper / chemistry*
Copper / metabolism
Crystallography, X-Ray / methods
Electron Transport
Ligands
Nitric Oxide / metabolism
Nitrites / metabolism
Oxidation-Reduction
Spectrum Analysis / methods
Stereoisomerism
Structure-Activity Relationship

Substances

Ligands
Nitrites
Azurin
Nitric Oxide
Copper