Abstract
Ygr203p, a 148-residue protein encoded by the ygr203w gene of Saccharomyces cerevisiae, is a homologue of the yeast Acr2 arsenate reductase encoded by the acr2 (or ypr200c) gene. It also shows significant sequence similarity to the human cell-cycle control Cdc25 phosphatase family. It has been overexpressed in soluble form in Escherichia coli with a His(6) tag at its C-terminus. The recombinant protein has been crystallized at 296 K using sodium chloride as precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 40.48, b = 50.95, c = 91.95 A. The asymmetric unit contains a monomer, giving a crystal volume per protein mass (V(m)) of 2.61 A(3) Da(-1) and a solvent content of 53.8%. The crystals diffract to better than 1.9 A resolution with Cu Kalpha X-rays. They are therefore suitable for high-resolution structure determination.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / chemistry*
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Arsenate Reductases
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Arsenite Transporting ATPases
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Crystallization
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Crystallography, X-Ray
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Fungal Proteins / biosynthesis
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Fungal Proteins / chemistry*
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Fungal Proteins / genetics
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Genetic Vectors / chemical synthesis
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Ion Pumps*
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Molecular Weight
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Multienzyme Complexes*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins
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Sequence Homology, Amino Acid
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cdc25 Phosphatases / chemistry
Substances
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Fungal Proteins
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Ion Pumps
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Multienzyme Complexes
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Recombinant Proteins
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Saccharomyces cerevisiae Proteins
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ARR2 protein, S cerevisiae
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Arsenate Reductases
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cdc25 Phosphatases
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Adenosine Triphosphatases
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Arsenite Transporting ATPases