C. elegans KLP-11/OSM-3/KAP-1: orthologs of the sea urchin kinesin-II, and mouse KIF3A/KIFB/KAP3 kinesin complexes

DNA Res. 2000 Apr 28;7(2):121-5. doi: 10.1093/dnares/7.2.121.

Abstract

Kinesins are intracellular multimeric transport motor proteins that move cellular cargo on microtubule tracks. It has been shown that the sea urchin KRP85/95 holoenzyme associates with a KAP115 non-motor protein, forming a heterotrimeric complex in vitro, called the Kinesin-II. Here we describe isolation of a cDNA clone corresponding to the klp-11 kinesin in C. elegans. Our sequence analysis of the encoded KLP-11 shows that it shares high homology with the OSM-3 kinesin. We also describe a nematode cDNA encoding KAP-1 that shares extensive homology with the sea urchin KAP115 kinesin associated protein. Sequence-based structural analysis of the OSM-3, KLP-11, and KAP-1, presented here suggests that these may form a heterotrimeric complex. We also describe the presence of a Drosophila armadillo consensus motif in CeKAP-1, first found in spKAP115, that suggests a possible role for the KAP-1 in signal transduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans Proteins*
  • Calcium-Binding Proteins / genetics
  • Carrier Proteins / genetics
  • Cloning, Molecular
  • Cytoskeletal Proteins*
  • Helminth Proteins / genetics*
  • Helminth Proteins / metabolism
  • Kinesins / genetics*
  • Kinesins / metabolism
  • Mice
  • Molecular Sequence Data
  • Muscle Proteins / genetics
  • Sea Urchins

Substances

  • Adaptor Proteins, Signal Transducing
  • Caenorhabditis elegans Proteins
  • Calcium-Binding Proteins
  • Carrier Proteins
  • Cytoskeletal Proteins
  • Helminth Proteins
  • KIFAP3 protein, human
  • Kif3a protein, mouse
  • Muscle Proteins
  • OSM-3 protein, C elegans
  • kinesin-II
  • Kifap3 protein, mouse
  • Kinesins