Abstract
Eukaryotic chromosome segregation depends on the mitotic spindle apparatus, a bipolar array of microtubules nucleated from centrosomes. Centrosomal microtubule nucleation requires attachment of gamma-tubulin ring complexes to a salt-insoluble centrosomal core, but the factor(s) underlying this attachment remains unknown. In budding yeast, this attachment is provided by the coiled-coil protein Spc110p, which links the yeast gamma-tubulin complex to the core of the yeast centrosome. Here, we show that the large coiled-coil protein kendrin is a human orthologue of Spc110p. We identified kendrin by its C-terminal calmodulin-binding site, which shares homology with the Spc110p calmodulin-binding site. Kendrin localizes specifically to centrosomes throughout the cell cycle. N-terminal regions of kendrin share significant sequence homology with pericentrin, a previously identified murine centrosome component known to interact with gamma-tubulin. In mitotic human breast carcinoma cells containing abundant centrosome-like structures, kendrin is found only at centrosomes associated with spindle microtubules.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens / chemistry*
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Breast Neoplasms / genetics
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Breast Neoplasms / metabolism
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Breast Neoplasms / pathology
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Calmodulin / metabolism*
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Calmodulin-Binding Proteins / chemistry
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Calmodulin-Binding Proteins / genetics
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Calmodulin-Binding Proteins / isolation & purification*
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Carcinoma / genetics
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Carcinoma / metabolism
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Carcinoma / pathology
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Centrosome / metabolism*
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Cytoskeletal Proteins
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Fibroblasts / metabolism
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Fungal Proteins / chemistry
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Gene Library
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Humans
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Microscopy, Fluorescence
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Molecular Sequence Data
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Neoplasm Proteins / chemistry
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Neoplasm Proteins / genetics
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Neoplasm Proteins / isolation & purification*
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Nuclear Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Species Specificity
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Spindle Apparatus / metabolism
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Tubulin / metabolism
Substances
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Antigens
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Calmodulin
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Calmodulin-Binding Proteins
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Cytoskeletal Proteins
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Fungal Proteins
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Neoplasm Proteins
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Nuclear Proteins
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Recombinant Fusion Proteins
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SPC110 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Tubulin
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kendrin
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pericentrin
Associated data
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GENBANK/AA000932
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GENBANK/AA333809
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GENBANK/AA600395
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GENBANK/AI970199
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GENBANK/U52962