Post-junctional mechanisms involved in the potentiation of cardiac adrenergic responses by cocaine

Eur J Pharmacol. 2000 May 26;397(1):139-50. doi: 10.1016/s0014-2999(00)00192-8.

Abstract

Cocaine cardiotoxicity is partly due to sympathetic activation of the heart resulting from inhibition of catecholamine uptake at the sympathetic nerve terminal and possible central sympathetic stimulation and/or inhibition. This study evaluated the role of postsynaptic mechanisms in potentiation by cocaine of cardiac adrenergic responses. Cardiovascular responses (arterial and left ventricular pressure, contractility and heart rate) to increasing doses of noradrenaline and to isoproterenol were obtained in anesthetized cats during a control period, after irreversible alpha-adrenoceptor blockade with phenoxybenzamine (5 mg/kg i.v.), and after cocaine (5 mg/kg, i.v.). Responses to noradrenaline were significantly reduced by phenoxybenzamine with lowering of the maximal rise of all parameters. Cocaine shifted the dose-response curve of noradrenaline to the left and enhanced its maximal effects. Some responses to isoproterenol, which is not taken up by nerve terminals, were also enhanced by cocaine. Pretreatment with chlorisondamine or verapamil prevented the cocaine-induced enhancement of the maximal response to noradrenaline and the response to isoproterenol, but it did not inhibit potentiation of submaximal doses. Lidocaine did not potentiate the response to noradrenaline or isoproterenol. Use of chlorisondamine instead of cocaine potentiated responses to all noradrenaline doses and enhanced the responses to isoproterenol. These results suggest that the potentiation by cocaine of cardiac responses to adrenergic stimuli involves presynaptic mechanisms to block noradrenaline re-uptake, and postsynaptic mechanisms to raise the maximal responses. The latter may result from inhibition of central sympathetic outflow or from activation of cardiac Ca(+) channels, leading to increased cardiac sensitivity to noradrenaline.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic alpha-Agonists / pharmacology
  • Adrenergic alpha-Antagonists / pharmacology
  • Animals
  • Blood Pressure / drug effects
  • Cats
  • Chlorisondamine / pharmacology
  • Cocaine / pharmacology*
  • Diastole
  • Dose-Response Relationship, Drug
  • Excitatory Postsynaptic Potentials / drug effects*
  • Female
  • Heart / drug effects*
  • Heart / physiology
  • Isoproterenol / pharmacology
  • Long-Term Potentiation / drug effects*
  • Male
  • Neuromuscular Junction / drug effects
  • Neuromuscular Junction / physiology
  • Norepinephrine / pharmacology
  • Phenoxybenzamine / pharmacology
  • Systole
  • Vasoconstrictor Agents / pharmacology*
  • Vasodilator Agents / pharmacology
  • Verapamil / pharmacology

Substances

  • Adrenergic alpha-Agonists
  • Adrenergic alpha-Antagonists
  • Vasoconstrictor Agents
  • Vasodilator Agents
  • Phenoxybenzamine
  • Verapamil
  • Cocaine
  • Chlorisondamine
  • Isoproterenol
  • Norepinephrine