Abstract
Flow cytometry, through the measurement of P-gp expression and function, is an important tool in the characterization of multidrug resistant cell lines used in pharmacology. Furthermore, as a complement to classical in vitro tests, this technique allows rapid evaluation of the capacity of new modulator agents to reverse the resistance to cytotoxic drugs and contributes a deeper insight into the mechanism of action of these new molecules.
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
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Antineoplastic Agents / metabolism
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Cell Cycle
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Doxorubicin / metabolism
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Drug Resistance, Multiple / physiology*
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Drug Resistance, Neoplasm / physiology*
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Flow Cytometry*
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Humans
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Neoplasm Proteins / metabolism*
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Phenotype
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Tumor Cells, Cultured / drug effects*
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Tumor Cells, Cultured / metabolism
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Antineoplastic Agents
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Neoplasm Proteins
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Doxorubicin