The application of single cell gel electrophoresis or Comet assay to human monitoring studies

Salud Publica Mex. 1999:41 Suppl 2:S109-13. doi: 10.1590/s0036-36341999000800007.

Abstract

Objective: In the search of new human genotoxic biomarkers, the single cell gel electrophoresis assay has been proposed as a sensible alternative.

Material and methods: This technique detects principally single strand breaks as well as alkali-labile and repair-retarded sites.

Results: Herein we present our experience using the single cell gel electrophoresis assay in human population studies, both occupationally and environmentally exposed.

Conclusions: We discuss the assay feasibility as a genotoxic biomarker.

MeSH terms

  • Biomarkers / analysis
  • Comet Assay*
  • Environmental Exposure*
  • Humans
  • Mexico
  • Monitoring, Physiologic
  • Occupational Exposure*
  • Urban Population

Substances

  • Biomarkers