Abstract
TIF1beta, a member of the transcriptional intermediary factor 1 family, has been reported to function as a corepressor for the large class of KRAB domain-containing zinc finger proteins of the Krüppel type. To address the biological function of TIF1beta, we have generated TIF1beta-deficient mice by gene disruption. TIF1beta protein was detected in wild-type but not TIF1beta(-/-) blastocysts. Homozygous mutant embryos, which developed normally until the blastocyst stage and underwent uterine implantation, were arrested in their development at the early egg-cylinder stage at about embryonic day (E) 5.5 and were completely resorbed by E8.5. Taken together, these results provide genetic evidence that TIF1beta is a developmental regulatory protein that exerts function(s) essential for early postimplantation development.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Alleles
-
Animals
-
Blastocyst / physiology
-
Blotting, Western
-
Brachyury Protein
-
Crosses, Genetic
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / physiology*
-
Embryo, Mammalian / metabolism
-
Embryo, Mammalian / physiology*
-
Fetal Proteins*
-
Fluorescent Antibody Technique
-
Gastrula / physiology
-
Genotype
-
In Situ Hybridization
-
Mice
-
Mice, Inbred C57BL
-
Mice, Transgenic
-
Mutagenesis
-
Nuclear Proteins*
-
Phenotype
-
Protein Structure, Tertiary
-
Repressor Proteins / genetics
-
Repressor Proteins / physiology*
-
Stem Cells
-
T-Box Domain Proteins / metabolism
-
Time Factors
-
Transcription Factors*
-
Tripartite Motif-Containing Protein 28
-
Zinc Fingers
Substances
-
DNA-Binding Proteins
-
Fetal Proteins
-
Nuclear Proteins
-
Repressor Proteins
-
T-Box Domain Proteins
-
Transcription Factors
-
Trim28 protein, mouse
-
Tripartite Motif-Containing Protein 28
-
Brachyury Protein