Abstract
A new CTX-M-type beta-lactamase (CTX-M-9) has been cloned from a clinical cefotaxime-resistant Escherichia coli strain. Despite the close identity that exists between the CTX-M-9 and Toho-2 beta-lactamases (88%), the 35 amino acids located between residues Ala-185 and Ala-219 are totally different in both enzymes. Outside of this region there are only six amino acids substitutions between both proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cefotaxime / metabolism
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Cefotaxime / pharmacology
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Cephalosporins / metabolism
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Cephalosporins / pharmacology
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Cloning, Molecular
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Escherichia coli Proteins*
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Hydrolysis
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Molecular Sequence Data
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Sequence Homology, Amino Acid
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Spain
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
Substances
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Cephalosporins
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Escherichia coli Proteins
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CTX-M-9 protein, E coli
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beta-Lactamases
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Cefotaxime