Molecular cloning of neuronally expressed mouse betaPix isoforms

Biochem Biophys Res Commun. 2000 Jun 16;272(3):721-5. doi: 10.1006/bbrc.2000.2845.

Abstract

Pix, a Pak-interacting exchange factor, is known to be involved in the regulation of Cdc42/Rac GTPases and Pak kinase activity. In this study, we cloned the cDNAs encoding two betaPix isoforms from mouse brain cDNA library. Both of the cloned genes, designated betaPix-b and betaPix-c (GenBank Accession Nos. AF247654 and AF247655, respectively), have a novel insert region consisting of 59 amino acid residues. In betaPix-c, 75 amino acid residues are deleted in the proline-rich region at the carboxyl-terminus of betaPix. In situ hybridization studies with insert region-specific probe in rat embryo show that insert region-containing isoforms are expressed mainly in the central nervous system. Moreover, temporal expression pattern of isoforms is correlated with the active neurogenesis period in the cerebral cortex and cerebellum. These results strongly suggest that betaPix isoforms may play important roles in the cellular events required for brain development such as neuronal migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing / genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / cytology
  • Brain / embryology
  • Brain / metabolism
  • Cell Cycle Proteins / analysis*
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / genetics*
  • Cell Movement
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Embryo, Mammalian / metabolism
  • Gene Expression Profiling
  • Guanine Nucleotide Exchange Factors*
  • In Situ Hybridization
  • Mice
  • Mice, Inbred ICR
  • Molecular Sequence Data
  • Molecular Weight
  • Neurons / cytology
  • Neurons / metabolism*
  • Protein Isoforms / analysis
  • Protein Isoforms / chemistry
  • Protein Isoforms / genetics
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Rats
  • Rats, Sprague-Dawley
  • Rho Guanine Nucleotide Exchange Factors
  • Sequence Deletion

Substances

  • Arhgef7 protein, mouse
  • Cell Cycle Proteins
  • DNA, Complementary
  • Guanine Nucleotide Exchange Factors
  • Protein Isoforms
  • RNA, Messenger
  • Rho Guanine Nucleotide Exchange Factors

Associated data

  • GENBANK/AF247654
  • GENBANK/AF247655