Facile synthesis of N-Fmoc-serine-S-GlcNAc: a potential molecular probe for the functional study of O-GlcNAc

Bioorg Med Chem Lett. 2000 Jun 5;10(11):1289-91. doi: 10.1016/s0960-894x(00)00223-7.

Abstract

A metabolically stable beta-N-acetylglucosaminyl-1-thio-N-Fmoc-serine (S-GlcNAc-Ser) derivative was synthesized in two procedures: one involving a coupling of a readily obtainable 1-pseudo-thiourea of GlcNAc (S-GlcNAc) and iodo-N-Boc-L-alanine benzyl ester, and the other utilizing a modified Mitsunobu reaction of GlcNAc-SH and a serine derivative.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetylglucosamine / chemistry*
  • Carbohydrate Conformation
  • Cysteine / analogs & derivatives*
  • Cysteine / chemical synthesis
  • Cysteine / chemistry
  • Molecular Probes / chemical synthesis*
  • Molecular Probes / chemistry*
  • Thioglucosides / chemical synthesis*
  • Thioglucosides / chemistry

Substances

  • 2-(benzyloxycarbonyl)-2-(fluorenylmethoxyamino)ethyl N-acetyl-3,4,5-tri-O-acetyl-2-acetamido-2-deoxy-1-thioglucopyranoside
  • Molecular Probes
  • Thioglucosides
  • Cysteine
  • Acetylglucosamine