Metalloproteinase inhibition reduces constrictive arterial remodeling after balloon angioplasty: a study in the atherosclerotic Yucatan micropig

Circulation. 2000 Jun 27;101(25):2962-7. doi: 10.1161/01.cir.101.25.2962.

Abstract

Background: Arterial remodeling after balloon angioplasty has been recognized as a major determinant of restenosis. Perturbation of collagen metabolism might be important. After balloon injury, matrix metalloproteinase (MMP) expression is upregulated. We investigated the effect of Batimastat, a nonspecific MMP inhibitor, on late lumen loss, arterial remodeling, and neointima formation after balloon dilation.

Methods and results: In atherosclerotic iliac arteries of 12 Yucatan micropigs, balloon dilation was performed, with intravascular ultrasound and quantitative angiography used before and after balloon dilation and at 42-day follow-up. The animals were randomly divided into 2 groups, the Batimastat group (n=6) and the vehicle group (n=6). All animals were intraperitoneally injected with either Batimastat or a vehicle immediately after balloon dilation and at 2 weeks and 4 weeks after balloon dilation. Angiographic and echographic late lumen loss in the Batimastat group versus the vehicle group was 0.3+/-0.1 versus 0.8+/-0.1 mm (P=0.01) and 2.2+/-0.5 versus 4.9+/-0.7 mm(2) (P=0.004), respectively. Late media-bounded area loss was used as a measure of remodeling after balloon dilation and was 0.9+/-0.6 mm(2) in the Batimastat group compared with 3.8+/-0.8 mm(2) in the vehicle group (P=0.003, mixed model analysis P=0.01). Neointima formation was 1.3+/-0.3 mm(2) in the Batimastat group and 1.0+/-0.2 mm(2) in the vehicle group (P=0. 542).

Conclusions: Metalloproteinase inhibition by Batimastat significantly reduced late lumen loss after balloon angioplasty by inhibition of constrictive arterial remodeling, whereas neointima formation was not inhibited by MMP inhibition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiography
  • Angioplasty, Balloon / adverse effects*
  • Animals
  • Arteriosclerosis / diagnosis
  • Arteriosclerosis / etiology*
  • Arteriosclerosis / metabolism
  • Arteriosclerosis / therapy*
  • Iliac Artery / diagnostic imaging
  • Iliac Artery / pathology
  • Iliac Artery / physiopathology*
  • Immunohistochemistry
  • Macrophages / pathology
  • Metalloendopeptidases / antagonists & inhibitors*
  • Metalloendopeptidases / metabolism
  • Phenylalanine / analogs & derivatives*
  • Phenylalanine / blood
  • Phenylalanine / therapeutic use
  • Postoperative Period
  • Protease Inhibitors / therapeutic use*
  • Swine
  • Swine, Miniature
  • Thiophenes / blood
  • Thiophenes / therapeutic use*
  • Tunica Intima / diagnostic imaging
  • Tunica Intima / pathology
  • Ultrasonography, Interventional

Substances

  • Protease Inhibitors
  • Thiophenes
  • Phenylalanine
  • batimastat
  • Metalloendopeptidases