Transdermal absorption of L-dopa from a new system composed of two separate layers of L-dopa and hydrogel in rats

Drug Dev Ind Pharm. 2000 Jul;26(7):755-9. doi: 10.1081/ddc-100101294.

Abstract

To maintain the stability of L-dopa in hydrogel, a new system composed of two separate layers of L-dopa and hydrogel was developed. L-Dopa sheets were made by immersing L-dopa solution into wiper sheets and by lyophilizing them. Examination for stability of L-dopa in the L-dopa sheet revealed that its stability was maintained for at least 12 weeks, providing the sheet was kept at room temperature in a dark box. In a cutaneous absorption study of L-dopa in rats, an L-dopa sheet was attached to the shaved abdominal skin. A hydrogel composed of cutaneous absorption enhancers, water and ethanol, was spread on vinyl tape (hydrogel sheet), and this sheet was placed over the L-dopa sheet. L-Dopa that was administered transdermally effectively penetrated through the skin: The plasma level of L-dopa peaked at 30 min and remained high between 60 and 180 min after the cutaneous application. Our system, composed of two separated layers of L-dopa and hydrogel, enabled the stability of L-dopa to be maintained without losing transdermal absorption of L-dopa.

MeSH terms

  • Administration, Cutaneous
  • Animals
  • Chemistry, Pharmaceutical
  • Chromatography, High Pressure Liquid
  • Dopamine Agents / administration & dosage*
  • Dopamine Agents / blood
  • Drug Stability
  • Hydrogel, Polyethylene Glycol Dimethacrylate*
  • Levodopa / administration & dosage*
  • Levodopa / blood
  • Male
  • Rats
  • Rats, Wistar
  • Skin Absorption*

Substances

  • Dopamine Agents
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Levodopa