Identification of Ehrlichia chaffeensis by nested PCR in ticks from Southern China

J Clin Microbiol. 2000 Jul;38(7):2778-80. doi: 10.1128/JCM.38.7.2778-2780.2000.

Abstract

A total of 717 ticks collected from southern China were examined by nested PCR for the presence of Ehrlichia chaffeensis. Sixteen (55. 2%) of 29 adult Amblyomma testudinarium ticks and 28 (11.7%) of 240 adult and at least 4.2% of 215 nymphal (pooled specimens) Haemaphysalis yeni ticks tested positive. Four other species of ticks were negative. Selected positive amplicons were confirmed by DNA sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arachnid Vectors / microbiology*
  • China
  • Cloning, Molecular
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • Ehrlichia chaffeensis / classification*
  • Ehrlichia chaffeensis / genetics*
  • Ehrlichia chaffeensis / isolation & purification
  • Molecular Sequence Data
  • Nymph / microbiology
  • Polymerase Chain Reaction / methods*
  • Sequence Analysis, DNA
  • Ticks / microbiology*

Substances

  • DNA, Bacterial

Associated data

  • GENBANK/AF147752