Abstract
C57BL/6 mice generate a vigorous H-2Db-restricted CTL response against murine leukemia virus (MuLV)-induced tumors. For many years it has been suggested that this response is directed to an MuLV-encoded peptide as well as to a nonviral tumor-associated peptide. Recently, a peptide from the leader sequence of gag was demonstrated to be the MuLV-derived epitope. Here we describe the molecular identification of the tumor-associated epitope. Furthermore, we show that the CTL response against this epitope can restrict the outgrowth of MuLV-induced tumors in vivo. The epitope is selectively presented by the MuLV-induced T cell tumors RBL-5, RMA, and MBL-2 as well as by the chemically induced T cell lymphoma EL-4. Intriguingly, these tumors share expression of the newly identified epitope because they represent variants of the same clonal tumor cell line, as evident from sequencing of the TCR alpha- and beta-chains, which proved to be identical. Our research shows that all sources of RBL-5, RMA, RMA-S, MBL-2, and EL-4 tumors are derived from a single tumor line, most likely EL-4.
MeSH terms
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Adoptive Transfer
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Animals
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Antigen Presentation*
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Binding Sites / immunology
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Cancer Vaccines / administration & dosage
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Cell Lineage / genetics
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Cell Lineage / immunology
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Epitopes, T-Lymphocyte / administration & dosage
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Epitopes, T-Lymphocyte / isolation & purification
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Epitopes, T-Lymphocyte / metabolism*
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Friend murine leukemia virus
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Gene Expression Regulation / immunology
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Genes, T-Cell Receptor beta
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Leukemia, Experimental / immunology
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Leukemia, Experimental / metabolism
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Leukemia, Experimental / pathology
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Lymphoma, T-Cell / immunology*
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Lymphoma, T-Cell / metabolism*
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Lymphoma, T-Cell / pathology
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Mice
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Mice, Inbred C57BL
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Mice, Nude
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Moloney murine leukemia virus
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Oligopeptides / administration & dosage
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Oligopeptides / chemical synthesis
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Oligopeptides / immunology
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Oligopeptides / metabolism
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Rauscher Virus
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Sequence Analysis, Protein
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T-Lymphocytes, Cytotoxic / immunology*
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T-Lymphocytes, Cytotoxic / metabolism*
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T-Lymphocytes, Cytotoxic / pathology
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T-Lymphocytes, Cytotoxic / transplantation
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Thymoma / immunology
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Thymoma / metabolism
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Thymoma / pathology
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Tumor Cells, Cultured / transplantation
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Tumor Virus Infections / immunology
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Tumor Virus Infections / metabolism
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Tumor Virus Infections / pathology
Substances
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Cancer Vaccines
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Epitopes, T-Lymphocyte
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Oligopeptides